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Water Analyses - Microbiology |
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Analytical Methods, Practical Detection Limits (PDL), Containers Preservation, and Holding Times for Selected Analytes
These bacteria analyses are recommended for drinking water, discharge water, swimming pools and spas. The holding times in the table below are regulatory limits. Standard Methods recommends 6 hours from collection to the start of the analysis. Chlorinated water must be collected in a container, which contains sodium thiosulfate.
| Determination |
Std. Methods 19th ed. 1995 |
PDL |
Container |
Preservation |
Holding Time Max. |
| Drinking Water |
| Total coliform: MF |
9222B |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| Total coliform: MTF |
9221B |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| Total coliform: PA |
9221D |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| Fecal coliform: MF |
9222D |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| Fecal coliform: MTF |
9221E |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| E. Coli |
9222G |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| E. Coli |
9223B |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| HPC |
9215B |
0/100mL |
Plastic/Glass |
Cool to 4°C |
30 Hours |
| BOD-5 |
5210B |
2 mg/L |
Plastic/Glass |
Cool to 4°C |
48 Hours |
| Surface Water |
| Fecal coliform: MF |
9222D |
0/100mL |
Plastic/Glass |
Cool to 4°C |
6/8 Hours(A) |
| E. Coli |
9223B(B) |
<1/100mL |
Plastic/Glass |
Cool to 4°C |
6/8 Hours(A) |
(A) - 6 hours from collection to the laboratory and 8 hours from collection to incubator, recommended and for regulatory use. 34 hours from collection to incubator for routine monitoring.
(B) - Using IDEXX Colilert Quanti-Tray procedure.
From Standard Methods for the Examination of Water and Waste Water, 19th edition, APHA, AWWA, and WEF, Washington, D.C., 1995.
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